Article
Positive and negative control of multidrug resistance by the Sit4 protein phosphatase in Kluyveromyces lactis.
The Journal of biological chemistry - 19 May 2000
Chen X J, Bauer B E, Kuchler K, Clark-Walker G D
Abstract excerpt
The nuclear gene encoding the Sit4 protein phosphatase was identified in the budding yeast Kluyveromyces lactis. K. lactis cells carrying a disrupted sit4 allele are resistant to oligomycin, antimycin, ketoconazole, and econazole but hypersensitive to paromomycin, sorbic acid, and 4-nitroquinoline-N-oxide (4-NQO). Overexpression of SIT4 leads to an elevation in resistance to paromomycin and to lesser extent...
Topics
- 4-Nitroquinoline-1-oxide
- Antifungal Agents
- Antimycin A
- Drug Resistance, Multiple
- Econazole
- Genetic Complementation Test
- Genotype
- Ketoconazole
- Kluyveromyces
- Molecular Sequence Data
- Oligomycins
- Paromomycin
- Phosphoprotein Phosphatases
- Protein Phosphatase 2
- Restriction Mapping
- Saccharomyces cerevisiae Proteins
- Sorbic Acid
- Tacrolimus