Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.

Abstract excerpt

An efficient method of constructing recombinant adenoviruses (Ads) has been established. The expression unit to be introduced into recombinant Ad was first inserted into the unique Swa I site of the full-length Ad genome cloned in a cassette cosmid. The cassette bearing the expression unit was then cotransfected into human embryonic kidney 293 cells together with the Ad DNA-terminal protein complex digested at...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Viral gastroenteritis research and epidemiology
• Share to Viral Infectious Diseases and Gene Expression in Insects