Article
Cloning and characterization of the UDP-sugar hydrolase gene (ushA) of Enterobacter aerogenes IFO 12010.
Biochemical and biophysical research communications - 16 Mar 2000
Lee K S, Song S B, Kim K E, Kim Y H, Kim S K, Kho B H, Ko D K, Choi Y K, Lee Y K, Kim C K, Kim Y C, Lim J Y, Kim Y, Min K H, Wanner B L
Abstract excerpt
A bacterial alkaline phosphatase (BAP, the phoA gene product) is primarily responsible for the hydrolysis of the substrates 5-bromo-4-chloro-3-indolylphosphate-p-toluidine (XP) and p-nitrophenyl phosphate (pNPP). Using these substrates and an E. coli phoA mutant, we have cloned Enterobacter aerogenes genes conferring an XP(+) phenotype. Two types of clones were identified based on phenotypic tests and DNA...
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