Article
Uracil uptake in Escherichia coli K-12: isolation of uraA mutants and cloning of the gene.
Journal of bacteriology - 1 Apr 1995
Andersen P S, Frees D, Fast R, Mygind B
Abstract excerpt
Mutants defective in utilization of uracil at low concentrations have been isolated and characterized. The mutations in question (uraA) map close to the upp gene encoding uracil phosphoribosyltransferase. By complementation analysis, a plasmid that complements the uraA mutation has been isolated. The uraA gene was shown to be the second gene in a bicistronic operon with upp as the promoter proximal gene. The...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Biological Transport, Active
- Carrier Proteins
- Chromosome Mapping
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
