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Article

Evidence for interactions between helices 5 and 8 and a role for the interdomain loop in tetracycline resistance mediated by hybrid Tet proteins.

The Journal of biological chemistry - 3 Mar 2000

Saraceni-Richards C A, Levy S B

Abstract excerpt

An interdomain hybrid Tet protein consisting of a class C alpha domain and a class B beta domain (Tet(C/B)) lacks detectable efflux ability and provides only minimal levels of resistance to tetracycline (Tc) (3 microg/ml) compared with intact class B (256 microg/ml) and class C (64 microg/ml). Twenty-one independently isolated mutants of the Tet(C/B) protein with increased Tc resistance were generated by random...

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Source

Identifiers

PMID: 10692399
DOI: 10.1074/jbc.275.9.6101

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evidence for interactions between helices 5 and 8 and a role for the interdomain loop in tetracycline resistance mediated by hybrid Tet proteins.PMID 10692399Read ↗

Interdomain hybrid Tet proteins confer tetracycline resistance only when they are derived from closely related members of the tet gene family.Semantic proximity Read ↗

Tet protein domains interact productively to mediate tetracycline resistance when present on separate polypeptides.Semantic proximity Read ↗

Importance of the GP dipeptide of the antiporter motif and other membrane-embedded proline and glycine residues in tetracycline efflux protein Tet(L).Semantic proximity Read ↗

Second-site suppressor mutations of inactivating substitutions at gly247 of the tetracycline efflux protein, Tet(B).Semantic proximity Read ↗

Genetic analysis of the tetA(C) gene on plasmid pBR322.Semantic proximity Read ↗

Twelve-transmembrane-segment (TMS) version (DeltaTMS VII-VIII) of the 14-TMS Tet(L) antibiotic resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity.Semantic proximity Read ↗

Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor.Semantic proximity Read ↗

Decreased function of the class B tetracycline efflux protein Tet with mutations at aspartate 15, a putative intramembrane residue.Semantic proximity Read ↗

Mechanism of Tet repressor induction by tetracyclines: length compensates for sequence in the alpha8-alpha9 loop.Semantic proximity Read ↗

Tet repressor-tetracycline interaction.Semantic proximity Read ↗

Structural investigation of Tet repressor loop 154-167: a time-resolved fluorescence study of three single Trp mutants.Semantic proximity Read ↗

Fe(2+)-tetracycline-mediated cleavage of the Tn10 tetracycline efflux protein TetA reveals a substrate binding site near glutamine 225 in transmembrane helix 7.Semantic proximity Read ↗

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