Article
Effects of activated ADP-ribosylation factors on Golgi morphology require neither activation of phospholipase D1 nor recruitment of coatomer.
The Journal of biological chemistry - 11 Feb 2000
Kuai J, Boman A L, Arnold R S, Zhu X, Kahn R A
Abstract excerpt
Nine mutations in the switch I and switch II regions of human ADP-ribosylation factor 3 (ARF3) were isolated from loss-of-interaction screens, using two-hybrid assays with three different effectors. We then analyzed the ability of the recombinant proteins to (i) bind guanine nucleotides, (ii) activate phospholipase D1 (PLD1), (iii) recruit coatomer (COP-I) to Golgi-enriched membranes, and (iv) expand and...
Topics
- ADP-Ribosylation Factors
- Animals
- CHO Cells
- Cell Line
- Coatomer Protein
- Cricetinae
- Enzyme Activation
- Fluorescent Antibody Technique
- Golgi Apparatus
- Guanosine 5'-O-(3-Thiotriphosphate)