Article
Identification of the magnesium ion binding site in the catalytic center of Escherichia coli primase by iron cleavage.
Biochemistry - 18 Jan 2000
Godson G N, Schoenich J, Sun W, Mustaev A A
Abstract excerpt
Magnesium is essential for the catalysis reaction of Escherichia coli primase, the enzyme synthesizing primer RNA chains for initiation of DNA replication. To map the Mg(2+) binding site in the catalytic center of primase, we have employed the iron cleavage method in which the native bound Mg(2+) ions were replaced with Fe(2+) ions and the protein was then cleaved in the vicinity of the metal binding site by...
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