Article
Dominant distribution of mitochondrial DNA from recipient oocytes in bovine embryos and offspring after nuclear transfer.
Journal of reproduction and fertility - 1 Jul 1999
Takeda K, Takahashi S, Onishi A, Goto Y, Miyazawa A, Imai H
Abstract excerpt
In the process of nuclear transfer, heteroplasmic sources of mitochondrial DNA from a donor cell and a recipient oocyte are mixed in the cytoplasm of the reconstituted embryo. The distribution of mitochondrial DNA heteroplasmy in nuclear transfer bovine embryos and resultant offspring was investigated by measuring polymorphism in the displacement loop region of mitochondrial DNA using PCR-mediated single-strand...
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