Article
Specific mutations in a viral RNA pseudoknot drastically change ribosomal frameshifting efficiency.
Proceedings of the National Academy of Sciences of the United States of America - 7 Dec 1999
Kim Y G, Su L, Maas S, O'Neill A, Rich A
Abstract excerpt
Many viruses regulate protein synthesis by -1 ribosomal frameshifting using an RNA pseudoknot. Frameshifting is vital for viral reproduction. Using the information gained from the recent high-resolution crystal structure of the beet western yellow virus pseudoknot, a systematic mutational analysis has been carried out in vitro and in vivo. We find that specific nucleotide tertiary interactions at the junction...
Topics
- Cells, Cultured
- Hydrogen Bonding
- Mutation
- Nucleic Acid Conformation
- RNA, Viral
- Ribosomes
- Structure-Activity Relationship