Article
Circulation of the plasma membrane in Dictyostelium.
Molecular biology of the cell - 1 Dec 1999
Aguado-Velasco C, Bretscher M S
Abstract excerpt
We have developed a fluorimetric assay with the use of the dye FM1-43 to determine the rate at which Dictyostelium amoebae endocytose their surface membrane. Our results show that they do so about once each 4-10 min. A clathrin null mutant takes its surface up only approximately 30% more slowly, showing that this membrane uptake cannot be caused by clathrin-coated vesicles. Surprisingly, Ax2 and its parent, NC4,...
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