Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Modification of human cytomegalovirus tropism through propagation in vitro is associated with changes in the viral genome.

The Journal of general virology - 1 Nov 1999

Sinzger C, Schmidt K, Knapp J, Kahl M, Beck R, Waldman J, Hebart H, Einsele H, Jahn G

Abstract excerpt

Following extensive propagation in fibroblasts, human cytomegalovirus (HCMV) loses tropism for a number of otherwise natural host cells, in particular, endothelial cells. In this study, the hypothesis was tested that loss of endothelial tropism is associated with the appearance of genomic variants. Initial quantitative focus expansion assays on endothelial monolayers demonstrated that, while the laboratory...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cells, Cultured
Share to Cytomegalovirus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cytomegalovirus and herpesvirus research

Share to DNA, Viral

Share to Endothelium, Vascular

Share to Genome, Viral

Share to Infant, Newborn

Share to Phenotype

Source

Identifiers

PMID: 10580048
DOI: 10.1099/0022-1317-80-11-2867

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Modification of human cytomegalovirus tropism through propagation in vitro is associated with changes in the viral genome.PMID 10580048Read ↗

Rescue of human cytomegalovirus strain AD169 tropism for both leukocytes and human endothelial cellsSemantic proximity Read ↗

Human Cytomegalovirus Tropism for Endothelial Cells: Not All Endothelial Cells Are Created EqualSemantic proximity Read ↗

In vitro selection of human cytomegalovirus variants unable to transfer virus and virus products from infected cells to polymorphonuclear leukocytes and to grow in endothelial cells.Semantic proximity Read ↗

Genomes of the endothelial cell-tropic variant and the parental Toledo strain of human cytomegalovirus are highly divergent.Semantic proximity Read ↗

Improvement of a quantitative cell tropism assay for rapid and reliable characterization of human cytomegalovirus mutants.Semantic proximity Read ↗

Two clinical isolates and the Toledo strain of cytomegalovirus contain endothelial cell tropic variants that are not present in the AD169, Towne, or Davis strains.Semantic proximity Read ↗

A review of genetic differences between limited and extensively passaged human cytomegalovirus strains.Semantic proximity Read ↗

Tropism of human cytomegalovirus for endothelial cells is determined by a post-entry step dependent on efficient translocation to the nucleus.Semantic proximity Read ↗

Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture.Semantic proximity Read ↗

Human cytomegalovirus: taking the strain.Semantic proximity Read ↗

Quantification of replication of clinical cytomegalovirus isolates in cultured endothelial cells and fibroblasts by a focus expansion assaySemantic proximity Read ↗

Stepwise adaptation of murine cytomegalovirus to cells of a foreign host for identification of host range determinants.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.