Article
Template secondary structure promotes polymerase jumping during PCR amplification.
BioTechniques - 1 Sept 1999
Viswanathan V K, Krcmarik K, Cianciotto N P
Abstract excerpt
Pairs of primers flanking known miniTn10 transposon insertion sites were used to confirm the presence of the transposon in DNA isolated from Legionella pneumophila mutants. It was expected that the polymerase chain reaction products derived from the mutant template would be larger than those from the wild-type (WT) template due to the presence of the 1.8-kb transposon. Instead, it was observed that the mutant...
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