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Article

Involvement of the sigmaN DNA-binding domain in open complex formation.

Molecular microbiology - 1 Aug 1999

Oguiza J A, Gallegos M T, Chaney M K, Cannon W V, Buck M

Abstract excerpt

sigmaN (sigma54) RNA polymerase holoenzyme closed complexes isomerize to open complexes in a reaction requiring nucleoside triphosphate hydrolysis by enhancer binding activator proteins. Here, we characterize Klebsiella pneumoniae sigmaN mutants, altered in the carboxy DNA-binding domain (F354A/F355A, F402A, F403A and F402A/F403A), that fail in activator-dependent transcription. The mutant holoenzymes have...

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Identifiers

PMID: 10447895
DOI: 10.1046/j.1365-2958.1999.01542.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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