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Article

Identification of multiple, differentially expressed messenger RNAs in dermal fibroblasts from patients with systemic sclerosis

1 Jul 1999

Abstract excerpt

OBJECTIVE: To simultaneously identify several genes whose expression is altered in dermal fibroblasts from patients with systemic sclerosis (SSc). METHODS: Total RNA was prepared from fibroblasts derived from clinically affected and unaffected skin of patients with SSc. The RNA samples were analyzed using differential-display reverse transcription-polymerase chain reaction (DDRT-PCR). Complementary DNA (cDNA)...

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PMID: 10403273
DOI: 10.1002/1529-0131(199907)42:7<1451::aid-anr19>3.0.co;2-6

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