Article
Isolation and properties of Enterococcus hirae mutants defective in the potassium/proton antiport system.
Journal of bacteriology - 1 Jul 1999
Kakinuma Y, Igarashi K
Abstract excerpt
A K+/H+ antiporter regulates cytoplasmic pH in Enterococcus hirae growing at alkaline pH. Mutants defective in this antiport activity were alkaline pH sensitive. One mutant, Pop1, lacked both K+/methylamine exchange at pH 9.5 and concomitant acidification of cytoplasmic pH. Pop1 grew well at pHs below 8 but did not at pHs above 9, conditions under which cytoplasmic pH was not fully acidified.
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