Article
Isolation of Enterococcus hirae mutant deficient in low-affinity potassium uptake at alkaline pH.
Bioscience, biotechnology, and biochemistry - 1 Jul 2002
Kawano Miyuki, Igarashi Kazuei, Kakinuma Yoshimi
Abstract excerpt
We here isolated an Enterococcus hirae mutant unable to grow well at pH 10. The influx rate calculated from steady-state 42K+/K+ exchange and the intracellular K+ concentration of the mutant were reduced to 53 and 55% of those of the wild-type, respectively. The activities of two high-affinity K+ uptake systems, KtrI and KtrII, were normal in the mutant, but the kinetics of net K+ uptake at pH 10 indicated that a...
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