Article
Inhibition of HIV replication by dominant negative mutants of Sam68, a functional homolog of HIV-1 Rev.
Nature medicine - 1 Jun 1999
Reddy T R, Xu W, Mau J K, Goodwin C D, Suhasini M, Tang H, Frimpong K, Rose D W, Wong-Staal F
Abstract excerpt
The HIV-1 Rev protein facilitates the nuclear export of mRNA containing the Rev response element (RRE) through binding to the export receptor CRM-1. Here we show that a cellular nuclear protein, Sam68 (Src-associated protein in mitosis), specifically interacts with RRE and can partially substitute for as well as synergize with Rev in RRE-mediated gene expression and virus replication. Differential sensitivity to...
Topics
- Adaptor Proteins, Signal Transducing
- Antibodies
- Carrier Proteins
- Cells, Cultured
- Chloramphenicol O-Acetyltransferase
- Cytoplasm
- DNA-Binding Proteins
- Enzyme Inhibitors
- Fatty Acids, Unsaturated
- Gene Expression Regulation, Viral
- Gene Products, rev
- Genes, Dominant