Article
Strand interruptions confer strand preference during intracellular correction of a plasmid-borne mismatch in Saccharomyces cerevisiae.
Current genetics - 1 Jun 1999
Yang Y, Kang X, Kohalmi L, Karthikeyan R, Kunz B A
Abstract excerpt
Site-directed mutagenesis was used to construct yeast centromere plasmids in which a strand nick or gap could be placed 5' or 3', on either strand, to a reporter gene (SUP4-o) carrying defined base mismatches. The plasmids were then transformed into yeast cells and the direction and efficiency of mismatch repair were assayed by scoring colouring of the transformant colonies. Strands that were nicked were...
Topics
- Base Pair Mismatch
- DNA Damage
- DNA Repair
- Deoxyribonuclease BamHI
- Deoxyribonuclease EcoRI
- Deoxyribonucleases, Type II Site-Specific
- Genetic Techniques
- Mutagenesis, Site-Directed
- Nucleic Acid Heteroduplexes
- Phenotype
- Plasmids
- Saccharomyces cerevisiae