Article
Continuous autotropic signaling by membrane-expressed tumor necrosis factor.
The Journal of biological chemistry - 18 Jun 1999
Haas E, Grell M, Wajant H, Scheurich P
Abstract excerpt
Tumor necrosis factor (TNF) exists in two bioactive forms, the membrane-integrated form and the proteolytically derived soluble cytokine. Cells that produce TNF are often responsive to TNF, allowing autocrine/juxtacrine feedback loops. However, whether the membrane form of TNF is involved in such regulatory circuits is unclear. Here we demonstrate that HeLa cells, expressing a permanently membrane-integrated...
Topics
- Amino Acid Chloromethyl Ketones
- Apoptosis
- Calcium-Calmodulin-Dependent Protein Kinases
- Caspases
- Cycloheximide
- Genes, Reporter
- HeLa Cells
- Humans
- Interferon-gamma
- Interleukin-6
- Membrane Proteins
- Mitogen-Activated Protein Kinases
- Mutation
- NF-kappa B
- Receptors, Tumor Necrosis Factor
- Signal Transduction
- Transfection
- Tumor Necrosis Factor-alpha