Article
Sequencing of two HLA-A blank alleles: implications in unrelated bone marrow donor matching.
Transplantation - 27 May 1999
Zanone-Ramseier R, Gratwohl A, Gmür J, Roosnek E, Tiercy J M
Abstract excerpt
BACKGROUND: DNA-based methods can type for HLA antigens with no or very low cell surface expression. The role of such serological blank antigens in bone marrow donor selection is unknown. METHODS: HLA-A serological blank antigens detected in two leukemic patients were cloned and sequenced from genomic DNA. mRNA expression and exon 3 splicing were determined by reverse transcriptase-polymerase chain reaction...
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