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Article

Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall.

Yeast (Chichester, England) - 1 Apr 1999

Pardo M, Monteoliva L, Pla J, Sánchez M, Gil C, Nombela C

Abstract excerpt

Protoplasts of Saccharomyces cerevisiae incubated in regenerating conditions secrete cell wall components in order to allow the biosynthesis of this structure. During the first hours of incubation, many of these are not retained in the forming cell wall but remain in the medium. We have developed a method for collecting the secreted proteins and have analysed these by two-dimensional electrophoresis to obtain a...

Topics

Amino Acid Sequence
Blotting, Western
Cell Wall
Culture Media, Conditioned
Electrophoresis, Gel, Two-Dimensional
Fungal Proteins
Genes, Fungal
Genetic Complementation Test
Glucuronidase
Glycoside Hydrolases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Glycosylation

Heat-Shock Proteins

Molecular Weight

Multienzyme Complexes

Mutation

Protoplasts

Saccharomyces cerevisiae

Sequence Analysis

Source

Identifiers

PMID: 10234784
DOI: 10.1002/(SICI)1097-0061(199904)15:6<459::AID-YEA387>3.0.CO;2-L

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