Article
Comparison of allelic ratios from paired blood and paraffin-embedded normal tissue for use in a polymerase chain reaction to assess loss of heterozygosity.
Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology - 1 Mar 1999
Zauber N P, Sabbath-Solitare M, Marotta S P, McMahon L, Bishop D T
Abstract excerpt
BACKGROUND: One method to assess loss of heterozygosity (LOH) of various genes is the amplification of DNA from neoplastic tissue by using microsatellite markers. LOH can best be considered on a quantitative basis as a comparison of allelic ratios of neoplastic tissue to that of the normal control. We will illustrate through quantitative methods the importance of using the appropriate controls when determining...
Topics
- Alleles
- Blood Cells
- Chromosomes, Human, Pair 5
- Colon
- DNA
- Densitometry
- Genetic Markers
- Humans
- Intestinal Mucosa
- Loss of Heterozygosity
- Microsatellite Repeats
- Paraffin Embedding
- Polymerase Chain Reaction