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Article

Imaging fluorescence resonance energy transfer between two green fluorescent proteins in living yeast.

FEBS letters - 19 Mar 1999

Sagot I, Bonneu M, Balguerie A, Aigle M

Abstract excerpt

We show that fluorescence resonance energy transfer between two mutants of the green fluorescent protein (GFP) can be monitored by imaging microscopy in living yeast. This work is based on the constitutive expression of a GFP-containing fusion protein and the inducible expression of the tobacco etch virus (TEV) protease. In the fusion protein, the P4.3 GFP mutant is linked to the YS65T GFP mutant by a spacer...

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PMID: 10218581
DOI: 10.1016/s0014-5793(99)00258-6

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