Article
Detection of various epitopes of murine osteopontin by monoclonal antibodies.
Biochemical and biophysical research communications - 2 Apr 1999
Hotta H, Kon S, Katagiri Y U, Tosa N, Tsukamoto T, Chambers A F, Uede T
Abstract excerpt
We immunized rats with recombinant murine osteopontin protein and obtained four monoclonal antibodies recognizing distinct epitopes of murine osteopontin. OPN1.2 recognized the amino-terminal half of OPN, while OPN2.2, OPN2.3, and OPN3.1 recognized the carboxy-terminal half of OPN. The epitope recognized by OPN2.2 was destroyed by further cleavage of the carboxy half of OPN. The epitope recognized by OPN2.3 was...
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