Article
Detection of various epitopes of murine osteopontin by monoclonal antibodies.
Biochemical and biophysical research communications - 2 Apr 1999
Hotta H, Kon S, Katagiri Y U, Tosa N, Tsukamoto T, Chambers A F, Uede T
Abstract excerpt
We immunized rats with recombinant murine osteopontin protein and obtained four monoclonal antibodies recognizing distinct epitopes of murine osteopontin. OPN1.2 recognized the amino-terminal half of OPN, while OPN2.2, OPN2.3, and OPN3.1 recognized the carboxy-terminal half of OPN. The epitope recognized by OPN2.2 was destroyed by further cleavage of the carboxy half of OPN. The epitope recognized by OPN2.3 was...
Topics
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Blotting, Western
- Calcification, Physiologic
- Epitopes
- Immunohistochemistry
- Mice
- Mice, Inbred BALB C
- Molecular Weight
- Mutation
- Myocardium
- Oligopeptides
- Osteopontin
- Peptide Fragments
- Protein Conformation
- Rats
- Recombinant Fusion Proteins