Article
Mutator phenotypes of yeast strains heterozygous for mutations in the MSH2 gene.
Proceedings of the National Academy of Sciences of the United States of America - 16 Mar 1999
Drotschmann K, Clark A B, Tran H T, Resnick M A, Gordenin D A, Kunkel T A
Abstract excerpt
Heterozygosity for germ-line mutations in the DNA mismatch repair gene MSH2 predisposes humans to cancer. Here we use a highly sensitive reporter to describe a spontaneous mutator phenotype in diploid yeast cells containing a deletion of only one MSH2 allele. We also identify five MSH2 missense mutations that have dominant mutator effects in heterozygous cells when expressed at normal levels from the natural MSH2...
Topics
- Base Pair Mismatch
- DNA Repair
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Heterozygote
- Humans
- MutS Homolog 2 Protein
- Mutation
- Phenotype
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins