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Article

Topology of the membrane domain of human erythrocyte anion exchange protein, AE1.

The Journal of biological chemistry - 5 Mar 1999

Fujinaga J, Tang X B, Casey J R

Abstract excerpt

Anion exchanger 1 (AE1) is the chloride/bicarbonate exchange protein of the erythrocyte membrane. By using a combination of introduced cysteine mutants and sulfhydryl-specific chemistry, we have mapped the topology of the human AE1 membrane domain. Twenty-seven single cysteines were introduced throughout the Leu708-Val911 region of human AE1, and these mutants were expressed by transient transfection of human...

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PMID: 10037758
DOI: 10.1074/jbc.274.10.6626

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