Isothermal digital detection of microRNA using background-free molecular circuit

Abstract excerpt

MicroRNA, a class of transcripts involved in the regulation of gene expression, are emerging as promising disease-specific biomarkers accessible from tissues or bodily fluids. However, their accurate quantification from biological samples remains challenging. We report a sensitive and quantitative microRNA method using an isothermal amplification chemistry adapted to a droplet digital readout. Building on molecula...

Topics

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• Biosensing Techniques
• Carcinoma, Non-Small-Cell Lung
• DNA
• Lung Neoplasms
• MicroRNAs
• Mutation
• Nucleic Acid Amplification Techniques
• Polymorphism, Single Nucleotide

Identifiers and source

Literature Corpus work

fe8efcc1-35c7-59f7-9f9b-d16441b4a7e9

DOI

10.1101/701276