Article

Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and whole rrn operon

2018-10-24

Abstract excerpt

<h4>Background: </h4> Profiling microbiome on low biomass samples is challenging for metagenomics since these samples are prone to present DNA from other sources, such as the host or the environment. The usual approach is sequencing specific hypervariable regions of the 16S rRNA gene, which fails to assign taxonomy to genus and species level. Here, we aim to assess long-amplicon PCR-based approaches for assigning...

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Literature Corpus work: fe556a7c-7d1e-5c87-adba-9156c1244772
DOI: 10.1101/450734

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