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Development of efficient methods for protoplast-to-plant regeneration in coffee and successful CRISPR-Cas9 editing in protoplasts

2026-04-23

Abstract excerpt

<title>Abstract</title> <p> Conventional coffee breeding is a long and complex process that limits genetic improvement, highlighting the need for advanced biotechnological tools. Protoplast-based CRISPR genome editing is a promising transgene-free approach, which enables precise genetic modifications and whole-plant regeneration while avoiding the stable integration of foreign DNA. However, reliable plant regene...

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Literature Corpus work
f9703730-20f2-5015-9876-02ca2058d85d
DOI
10.21203/rs.3.rs-9347124/v1
Open publication

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Development of efficient methods for protoplast-to-plant regeneration in coffee and successful CRISPR-Cas9 editing in protoplastsDOI 10.21203/rs.3.rs-9347124/v1
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