Article
Improved Transformation and Regeneration of Indica Rice: Disruption of SUB1A as a Test Case via CRISPR-Cas9.
International journal of molecular sciences - 29 Jun 2021
Liang Yuya, Biswas Sudip, Kim Backki, Bailey-Serres Julia, Septiningsih Endang M
Abstract excerpt
Gene editing by use of clustered regularly interspaced short palindromic repeats (CRISPR) has become a powerful tool for crop improvement. However, a common bottleneck in the application of this approach to grain crops, including rice (Oryza sativa), is efficient vector delivery and calli regeneration, which can be hampered by genotype-dependent requirements for plant regeneration. Here, methods for...
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