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Active Nuclear Shuttling Enables Efficient Virus-Free CAR Gene Integration Using Ready-to-Use Lipid Nanoparticles

2026-06-02

Abstract excerpt

Non-viral engineering of chimeric antigen receptor T (CAR-T) cells is highly desirable to overcome the cost, safety, and scalability limitations associated with viral vectors and electroporation. However, efficient nuclear delivery and stable genomic integration of DNA in primary human T cells remain major challenges. Here, we established a virus-free CAR-T manufacturing platform using lipid nanoparticles (LNPs) c...

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Literature Corpus work: f95140ea-2b4a-58ec-b2aa-3a74a7694a28
DOI: 10.64898/2026.05.29.728914

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