Article

In-Silico Evaluation and Selection of the Best 16S rRNA Gene Primers for Use in Next-Generation Sequencing to Detect Oral Bacteria and Archaea.

2021-05-24

Abstract excerpt

<title>Abstract</title> <p><bold>Background:</bold> Sequencing has been widely used to study the composition of the oral microbiome present in various health conditions. The extent of the coverage of the 16S rRNA gene primers employed for this purpose has not, however, been evaluated in silico using oral-specific databases. This paper analyses these primers using two databases containing 16S rRNA sequences from b...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: fd0e65a3-d3de-5aff-a55b-b8e9134c8673
DOI: 10.21203/rs.3.rs-516961/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

In-Silico Evaluation and Selection of the Best 16S rRNA Gene Primers for Use in Next-Generation Sequencing to Detect Oral Bacteria and Archaea.DOI 10.21203/rs.3.rs-516961/v1Read

Impact of 16S rRNA gene redundancy and primer pair selection on the quantification and classification of oral microbiota in next-generation sequencingSemantic proximity Read

OTUs clustering should be avoided for defining oral microbiomeSemantic proximity Read

Comparative Analysis of Primers Used for 16S rRNA Gene Sequencing in Oral Microbiome StudiesSemantic proximity Read

The Human Oral MicrobiomeSemantic proximity Read

Profiling of Oral Bacterial CommunitiesSemantic proximity Read

Evaluation of an effective detection and quantification method for particular microorganisms by comparing NGS-based metagenome profiling dataSemantic proximity Read

Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and databaseSemantic proximity Read

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies.Semantic proximity Read

Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and databaseSemantic proximity Read

The Impact of Primer Design on Amplicon-Based Metagenomic Profiling Accuracy: Detailed Insights into Bifidobacterial Community StructureSemantic proximity Read

Evaluating and Improving SSU rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean SurveysSemantic proximity Read

CORE: A Phylogenetically-Curated 16S rDNA Database of the Core Oral MicrobiomeSemantic proximity Read