Article

Amplicon Sequence Variant-Based Oral Microbiome Analysis Using QIIME 2

2020-08-08

Abstract excerpt

The bacterial composition of oral samples has traditionally been determined by PCR amplicon sequencing of 16S rRNA genes. Recent amplicon sequence variant (ASV)-based analyses of 16S rRNA genes differ from that based on operational taxonomic unit (OTU) clustering in the way it deals with sequences having potential errors. However, little information is available on its application in oral microbiome studies. Here,...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d76596cc-750b-5faa-aa42-5271d833749b
DOI: 10.20944/preprints202008.0206.v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Amplicon Sequence Variant-Based Oral Microbiome Analysis Using QIIME 2DOI 10.20944/preprints202008.0206.v1Read

OTUs clustering should be avoided for defining oral microbiomeSemantic proximity Read

Profiling of Oral Bacterial CommunitiesSemantic proximity Read

Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and databaseSemantic proximity Read

Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and databaseSemantic proximity Read

High-resolution ISR amplicon sequencing reveals personalized oral microbiome.Semantic proximity Read

The Influences of Bioinformatics Tools and Reference Databases in Analyzing the Human Oral Microbial CommunitySemantic proximity Read

Comparative Analysis of Primers Used for 16S rRNA Gene Sequencing in Oral Microbiome StudiesSemantic proximity Read

Microbiome Analysis Using 16S Amplicon Sequencing: From Samples to ASVs.Semantic proximity Read

CORE: A Phylogenetically-Curated 16S rDNA Database of the Core Oral MicrobiomeSemantic proximity Read

In-Silico Evaluation and Selection of the Best 16S rRNA Gene Primers for Use in Next-Generation Sequencing to Detect Oral Bacteria and Archaea.Semantic proximity Read

The salivary microbiome as a diagnostic biomarker of periodontitis: a 16S multi-batch study before and after the removal of batch effectsSemantic proximity Read

Microbial characteristics of the DMFT index in a high HIV prevalence settingSemantic proximity Read