Article

Simultaneous detection and identification of pathogenic Cronobacter species by high‐resolution melting analysis in powdered infant formulas

2018-02-01

Abstract excerpt

A combination method of real‐time PCR and high‐resolution melting (HRM) analysis for the rapid detection and specific classification of six pathogenic Cronobacter species based on gene cgcA was developed. HRM profiles with distinct Tₘ (melting temperature) peaks were consistently obtained with each species, represented by a single peak ranging from 86.02 to 86.80 °C. The detection limit ranged from 0.1 to 1 pg per...

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Literature Corpus work: f5b797dc-aa60-53f4-89d4-e874adc58d9a
DOI: 10.1111/1471-0307.12410

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