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Analysis of Salivary Bacterial Community by Direct PCR and High Resolution Melting Curve and Its Forensic Applications.

Fa yi xue za zhi - 25 Dec 2022

Yang Rui, Chen Jiong, Zhao Gui-Sen

Abstract excerpt

OBJECTIVES: To develop a rapid test for salivary bacterial community based on direct PCR (dPCR) and high resolution melting (HRM) curve analysis, to evaluate its application value in forensic medicine. METHODS: The salivary bacteria were collected by centrifugation and then resuspended in Tris-EDTA (TE) buffer, and directly used as the template for amplification and HRM curve analysis (dPCR-HRM) of the 16S rDNA...

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PMID: 36914386
DOI: 10.12116/j.issn.1004-5619.2021.511006

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