Article

Spatial mapping of proteins and their activity states in cancer models by multiplex in situ PLA

2025-07-11

Abstract excerpt

Improved methods are needed to gain insights in how proteins exert their myriad roles in cells and organs. Multiplex in situ proximity ligation assay (misPLA), described herein, can provide a window into the functional states of proteins in cells and tissues by applying pairs of antibody–oligonucleotide conjugates to generate amplifiable DNA circles upon proximal binding. The analysis reveals interactions and modi...

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Identifiers and source

Literature Corpus work: f132e977-a9db-5535-9e87-9773a4d484d3
DOI: 10.1101/2025.07.11.662357

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