Article

Systematic determination of the mitochondrial proportion in human and mice tissues for single-cell RNA sequencing data quality control

2020-02-22

Abstract excerpt

<h4>Motivation</h4> Quality control (QC) is a critical step in single-cell RNA-seq (scRNA-seq) data analysis. Low-quality cells are removed from the analysis during the QC process to avoid misinterpretation of the data. One of the important QC metrics is the mitochondrial proportion (mtDNA%), which is used as a threshold to filter out low-quality cells. Early publications in the field established a threshold of 5...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e576ad4c-9004-5435-bdd1-9dc6e16f2291
DOI: 10.1101/2020.02.20.958793

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Systematic determination of the mitochondrial proportion in human and mice tissues for single-cell RNA sequencing data quality controlDOI 10.1101/2020.02.20.958793Read

Single-cell mitochondrial sequencing reveals low-frequency mitochondrial mutations in naturally aging mice.Semantic proximity Read

A bioinformatics pipeline for identifying homoplasmic and heteroplasmic mitochondrial DNA SNVs in single-cell RNA-Seq datasets.Semantic proximity Read

Optimized Methods for Obtaining scRNA-seq and snRNA-seq Data from Skeletal Muscle Stored in Nucleic Acid Stabilizing PreservativeSemantic proximity Read

Benchmarking Single-Cell RNA Sequencing Protocols for Cell Atlas ProjectsSemantic proximity Read

Filtering cells with high mitochondrial content removes viable metabolically altered malignant cell populations in cancer single-cell studiesSemantic proximity Read

Biology-inspired data-driven quality control for scientific discovery in single-cell transcriptomicsSemantic proximity Read

<i>MALAT1</i> expression indicates cell quality in single-cell RNA sequencing dataSemantic proximity Read

Single-Nucleus RNA-Sequencing Profiling of Mouse Lung. Reduced Dissociation Bias and Improved Rare Cell-Type Detection Compared with Single-Cell RNA SequencingSemantic proximity Read

Single-cell Individual Complete mtDNA Sequencing Uncovers Hidden Mitochondrial Heterogeneity in Human and Mouse OocytesSemantic proximity Read

Exploring Heteroplasmic Variants in mtDNA: Insights from Single-Cell TranscriptomicsSemantic proximity Read

Filtering cells with high mitochondrial content depletes viable metabolically altered malignant cell populations in cancer single-cell studiesSemantic proximity Read

Exploring Heteroplasmic Variants in mtDNA: Insights from Single-Cell Transcriptomics.Semantic proximity Read