Article

Epigenetic modification and characterization of the <i>MGMT</i> promoter region using CRISPRoff in glioblastoma cells

2023-09-22

Abstract excerpt

The methylation status of the O6-methylguanine DNA methyltransferase ( MGMT ) promoter region is a critical predictor of response to alkylating agents in glioblastoma. However, current approaches to study the MGMT status focus on analyzing models with non-identical backgrounds. Here, we present an epigenetic editing approach using CRISPRoff to introduce site-specific CpG methylation in the MGMT promoter region...

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Literature Corpus work: e1f6119d-6f5e-5440-ba0d-d9386aef4da4
DOI: 10.1101/2023.09.21.558779

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