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Molecular cloning and detection of chromosomal and extrachromosomal DNA of the mycoplasmalike organism associated with little leaf disease in periwinkle (Catharanthus roseus)

1990-09-01

Abstract excerpt

Preparations enriched for periwinkle little leaf mycoplasmalike organism (MLO) were obtained by a method involving isolation of sieve cells from diseased plants of periwinkle (Catharanthus roseus). DNA was extracted from the preparations, ligated into plasmids pSP64 and pSP65, and cloned in Escherichia coli JM83. Selected recombinant plasmids and cloned DNA inserts were labeled with biotin and employed as probes i...

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Literature Corpus work
e1a5fc56-2205-531c-816a-380a6a9034fb
DOI
10.1094/phyto-80-789
Open publication

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Molecular cloning and detection of chromosomal and extrachromosomal DNA of the mycoplasmalike organism associated with little leaf disease in periwinkle (Catharanthus roseus)DOI 10.1094/phyto-80-789
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