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Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene

1992-08-01

Abstract excerpt

A polymerase chain reaction (PCR) method has been developed with a 558-bp fragment of the 16S rRNA gene as template DNA and two oligonucleotide primers from conserved regions of this gene. The suitability of the system has been tested with 17 isolates of mycoplasmalike organisms (MLOs) maintained in periwinkle (Catharanihus roseus) and with nine MLO samples from field-grown woody plants. With DNA preparations enri...

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Identifiers and source

Literature Corpus work
3aee5358-9622-5132-9d8b-3c7ff41a6470
DOI
10.1094/phyto-82-828
Open publication

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