Article

Isolation and cloning of DNA fragments from a mycoplasmalike organism associated with walnut witches'-broom disease

1992-03-01

Abstract excerpt

Total DNA was extracted using CTAB buffer from freeze-dried leaves of mature walnut (Juglans nigra) showing severe symptoms of walnut witches'-broom (WWB) disease. DNA of WWB mycoplasmalike organism (MLO) was separated from host DNA by CsCl/bisbenzimide gradient centrifugation. A genomic library was constructed from MLO DNA that contained either chromosomal or extrachromosomal DNA fragments. Extrachromosomal DNA p...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 7e9925ef-b859-5bee-bde7-0f458e0afb4f
DOI: 10.1094/phyto-82-306

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Isolation and cloning of DNA fragments from a mycoplasmalike organism associated with walnut witches'-broom diseaseDOI 10.1094/phyto-82-306Read

Development and application of cloned DNA probes for a mycoplasmalike organism associated with sweetpotato witches'-broomSemantic proximity Read

Phylogenetic diversity of phytopathogenic mycoplasmalike organisms.Semantic proximity Read

Genetic relatedness of mycoplasmalike organisms affecting elm, adler, and ash in Europe and North AmericaSemantic proximity Read

First Report of ‘Candidatus Phytoplasma asteris’ Subgroup 16SrI-D Associated with Rosa xanthina Witches’ Broom in ChinaSemantic proximity Read

Association of a Phytoplasma with Pistachio Witches' Broom Disease in IranSemantic proximity Read

Molecular identification and characterization of a new phytoplasma strain associated with Chinese chestnut yellow crinkle disease in ChinaSemantic proximity Read

Molecular cloning and detection of chromosomal and extrachromosomal DNA of the mycoplasmalike organism associated with little leaf disease in periwinkle (Catharanthus roseus)Semantic proximity Read

DNA probes and PCR primers for the detection of a phytoplasma associated with peanut witches'-broom.Semantic proximity Read

Molecular Characterization of a Phytoplasma Associated with Potato Witches'-broom Disease in IranSemantic proximity Read

Molecular Characterization of Phytoplasma Associated with Rose Witches'-Broom in ChinaSemantic proximity Read

First Report of ‘Candidatus Phytoplasma ziziphi’ Subgroup 16SrV-B Associated with Prunus salicina Witches’-Broom in ChinaSemantic proximity Read

First Report of Aster Yellows Phytoplasma (16SrI‐B) Associated with Witches' Broom Disease of Melia azedarach var. japonica in KoreaSemantic proximity Read