Article

Two-target quantitative PCR to predict library composition for shallow shotgun sequencing

2020-09-21

Abstract excerpt

Shotgun sequencing enables retrieving high resolution information 40 from complex microbial communities. However, the technique is limited by missing information about host-to-microbe ratios observed in different sample types. This makes it challenging to plan sequencing experiments, especially in the context of high sample multiplexing and/or limited sequencing output. We evaluated a qPCR-based assay to predict h...

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Literature Corpus work: df65f516-f666-5e04-b11b-77d89ae8aa2c
DOI: 10.1101/2020.09.21.304006

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