Article

PCR analysis of insertion sequences leads to the generation of artefact amplicons.

2026-02-24

Abstract excerpt

Insertion Sequences (ISs) are small self-mobilizing DNA elements widespread across prokaryotic genomes, including chromosomes and plasmids. IS elements frequently colocalize with antibiotic resistance genes and mediate their mobilization, often as part of larger genomic structures which encompass multiple IS elements and antibiotic resistance genes. In this study, we employed PCR to amplify DNA sequences containin...

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Literature Corpus work: d73693c0-b140-5ae3-a219-8dea2aedcfac
DOI: 10.1099/acmi.0.001191.v1

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