Article
Factors determining the frequency of plasmid cointegrate formation mediated by insertion sequence IS3 from Escherichia coli.
Molecular & general genetics : MGG - 1 May 1991
Spielmann-Ryser J, Moser M, Kast P, Weber H
Abstract excerpt
Transposition events mediated by plasmidborne copies of the insertion sequence IS3 of Escherichia coli are difficult to detect because of a low frequency of cointegrate formation. We found that cointegration activity could be strongly enhanced by using plasmid constructions in which a second IS3 element, disabled by a large deletion, was placed adjacent to an intact IS3 copy. Attempts to construct plasmids...
Topics
- Base Sequence
- Chromosomes, Bacterial
- DNA Transposable Elements
- Escherichia coli
- Genes, Bacterial
- Methylation
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Open Reading Frames
- Plasmids