Article

Alignment-Free Guided Design of a Pan-Orthoflavivirus RT-qPCR Assay

2026-03-20

Abstract excerpt

The co-circulation and rapid expansion of the genus Orthoflavivirus , including dengue virus (DENV), Zika (ZIKV), and Japanese encephalitis virus (JEV), pose significant global health challenges. Developing inclusive pan-genus molecular diagnostics is hindered by high nucleotide divergence (>25%–30%) and the computational limitations of traditional multiple sequence alignment in detecting conserved motifs across...

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Literature Corpus work: d0bc9df7-1a5b-5022-a3c6-cc97040199c1
DOI: 10.64898/2026.03.17.712358

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