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Article

A Real-Time RT-PCR Method for the Universal Detection and Identification of Flaviviruses

17 Nov 2007

Abstract excerpt

Here we describe an optimized molecular protocol for the universal detection and identification of flaviviruses. It combines the convenient real-time polymerase chain reaction (PCR) format with a broad spectrum of flavivirus detection. This assay, based on the amplification of a 269-272 nt (depending on the flavivirus tested) region at the N terminal end of the NS5 gene, enabled the amplification of 51 flavivirus...

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Identifiers

PMID: 18020965
DOI: 10.1089/vbz.2007.0206

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