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A Serotype-Specific and Multiplex PCR Method for Whole-Genome Sequencing of Dengue Virus Directly from Clinical Samples.

Microbiology spectrum - 26 Oct 2022

Su Wenzhe, Jiang Liyun, Lu Weizhi, Xie Huaping, Cao Yimin, Di Biao, Li Yan, Nie Kai, Wang Huanyu, Zhang Zhoubin, Xu Songtao

Abstract excerpt

Dengue virus (DENV) is the most globally prevalent member of the genus Flavivirus in the family Flaviviridae, which can be classified into four serotypes. Historically, molecular epidemiological studies of DENV depended on E gene sequencing. The development of next-generation sequencing (NGS) allowed its application to viral whole-genome sequencing (WGS). In this study, we report the improvement of the existing...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 36094197
DOI: 10.1128/spectrum.01210-22
PMCID: PMC9602986

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