Article

Exposure to graphene oxide sheets alters the expression of reference genes used for real-time RT-qPCR normalization

2018-11-14

Abstract excerpt

<h4>Background</h4> studies that unravel the interactions between thin, 2D graphene oxide (GO) sheets and the biological milieu, including cells and tissues, are multiplying quickly as the biomedical applications of those and other 2D materials continue to be explored. Many of such studies rely on real-time RT-qPCR as a powerful, yet relatively simple technique to determine gene expression. However, a systematic...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c5496686-e720-5a50-b4dc-5242f0e74c15
DOI: 10.1101/469304

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Exposure to graphene oxide sheets alters the expression of reference genes used for real-time RT-qPCR normalizationDOI 10.1101/469304Read

Selecting Suitable Reference Genes for qPCR Normalization: A Comprehensive Analysis in MCF-7 Breast Cancer Cell LineSemantic proximity Read

Identification of stable reference genes for quantitative real-time PCR in human fibroblasts from lymph nodes and synoviumSemantic proximity Read

RNA-Seq is not required to determine stable reference genes for qPCR normalizationSemantic proximity Read

Reference gene validation for qPCR on normoxia- and hypoxia-cultured human dermal fibroblasts exposed to UVA: Is β-actin a reliable normalizer for photoaging studies?Semantic proximity Read

Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell lineSemantic proximity Read

Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineeringSemantic proximity Read

Reference genes in real-time PCR.Semantic proximity Read

Acute Exposure of Upcyte® Hepatocytes to Sub-lethal Concentrations of Graphene Oxide: Impairment of Phase-I Xenobiotic Metabolism and Albumin TranscriptionSemantic proximity Read

The Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem CellsSemantic proximity Read

RNA-seq reveals more consistent reference genes for gene expression studies in human non-melanoma skin cancersSemantic proximity Read

Reference genes for mesangial cell and podocyte qPCR gene expression studies under high-glucose and renin-angiotensin-system blocker conditionsSemantic proximity Read

Normalization of Gene Expression for Acute Leukemia Studies: Selecting a Set of Endogenous Genes ControlSemantic proximity Read