Article

RNA-seq reveals more consistent reference genes for gene expression studies in human non-melanoma skin cancers

2016-08-02

Abstract excerpt

Identification of appropriate reference genes (RGs) is critical to accurate data interpretation in quantitative real-time PCR (qPCR) experiments. In this study, we have utilised next generation RNA-sequencing (RNA-seq) to analyse the transcriptome of a panel of non-melanoma skin cancer lesions, identifying genes, which are consistently expressed across all samples. Genes encoding ribosomal proteins were amongst th...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 7b2ded16-71d2-5145-99f5-8d94a2e6f022
DOI: 10.7287/peerj.preprints.2331v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

RNA-seq reveals more consistent reference genes for gene expression studies in human non-melanoma skin cancersDOI 10.7287/peerj.preprints.2331v1Read

Genome Based Search for Reference Genes for Gene Expression Analysis in Oral Cancer: a Data Science Driven ApproachSemantic proximity Read

Identification of stable reference genes for quantitative real-time PCR in human fibroblasts from lymph nodes and synoviumSemantic proximity Read

Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell linesSemantic proximity Read

Validation of Reference Genes for Oral Cancer Detection Panels in a Prospective Blinded Cohort.Semantic proximity Read

Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell lineSemantic proximity Read

Selecting Suitable Reference Genes for qPCR Normalization: A Comprehensive Analysis in MCF-7 Breast Cancer Cell LineSemantic proximity Read

Evaluation of reference genes suitable for studying mRNAs and microRNAs expression in thyroid neoplasmsSemantic proximity Read

HRT Atlas v1.1 database: redefining human and mouse housekeeping genes and candidate reference transcripts by mining massive RNA-seq datasetsSemantic proximity Read

Selection of Ideal Reference Genes for Gene Expression Analysis in COVID-19 and MucormycosisSemantic proximity Read

Research Techniques Made Simple: Methodology and Clinical Applications of RNA Sequencing.Semantic proximity Read

Selection of appropriate reference genes for normalization of qRT-PCR based gene expression analysis in SARS-CoV-2, and Covid-associated Mucormycosis infectionSemantic proximity Read

Identification of stable reference genes for quantitative PCR in koalasSemantic proximity Read