Article

In Vivo Evolution of Monoclonal Antibody CR3022 to Achieve Cross-Neutralization of SARS-CoV-2 and Implications for Vaccine Strategies Against SARS-Related Viruses

2025-08-06

Abstract excerpt

The epitope that monoclonal CR3022 binds to represents a promising target for broad protection against a wide range of human and zoonotic coronaviruses. We developed a powerful model to evaluate antibody affinity maturation in vivo using immunoglobulin (Ig)-humanized mice that express the predicted germline heavy chain of antibody CR3022. SARS-CoV/SARS-CoV-2 sequential immunization led to the convergent evolution...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c4609475-c47a-5644-a010-84490bf0140f
DOI: 10.1101/2025.08.05.666673

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

In Vivo Evolution of Monoclonal Antibody CR3022 to Achieve Cross-Neutralization of SARS-CoV-2 and Implications for Vaccine Strategies Against SARS-Related VirusesDOI 10.1101/2025.08.05.666673Read

Broadening a SARS-CoV-1–neutralizing antibody for potent SARS-CoV-2 neutralization through directed evolutionSemantic proximity Read

Therapeutic Potential of SARS-CoV-2-Specific Monoclonal Antibody CR3022Semantic proximity Read

Somatic evolution of a cross-reactive germline antibody that expands its breadth to neutralize new SARS-CoV-2 variantsSemantic proximity Read

Exploring the role of framework mutations in enabling breadth of a cross-reactive antibody (CR3022) against the SARS-CoV-2 RBD and its variants of concern.Semantic proximity Read

Directed evolution of a stem-helix–targeting antibody enables MERS-CoV cross-neutralization through enhanced binding affinitySemantic proximity Read

Optimizing the breadth of SARS-CoV-2-neutralizing antibodies in vivo and in silicoSemantic proximity Read

A Monoclonal Antibody Panel to Track Ongoing Antigenic Evolution of SARS-CoV-2 VariantsSemantic proximity Read

The prominent role of a CDR1 somatic hypermutation for convergent IGHV3-53/3-66 antibodies in binding to SARS-CoV-2Semantic proximity Read

Affinity maturation of cross-reactive CR3022 antibody against the receptor binding domain of SARS-CoV-2 via in silico site-directed mutagenesisSemantic proximity Read

Potent SARS-CoV-2 binding and neutralization through maturation of iconic SARS-CoV-1 antibodiesSemantic proximity Read

A human antibody that broadly neutralizes betacoronaviruses protects against SARS-CoV-2 by blocking the fusion machinerySemantic proximity Read

Dissecting the impact of somatic hypermutation on SARS-CoV-2 neutralization and viral escapeSemantic proximity Read